add 100ul Resazurin
cap the tubes--make sure to get the petroleum stopper top a bit wet so that when you close the screw top on it, it can rotate and fully tighten down.
bring water bath to rolling boil
place 9 tubes in boiling water for 10m
while tubes are still hot, sparge with N2, while bring tubes down to rt with water bath.
wait until following day to see if any tubes are leaking (based on indicator color change clear-> pink
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