lab meeting outline

try and make new figure showing location among screen hits of the hypoxia hits with oe v 2 on x axis and 2 v 1 on y axis. emphasize that the majority of the transcription factors are not in inducing conditions when screened, whereas the hypoxia genes are.

you should also see where cell cycle genes fall and see if you can add this to the plot.

note: this will require you go through the images and say for sure which ones are expressed.

data on Rox1-YFP in HEM1::LEU2 background. Contrast with previous results. Explain that the major difference between two experiments was 1) plating OD 2) normalized time in imaging media. I conclude that the previously observed noise characteristics may be due to observing Rox1-YFP expression at different stages in progression to heme-starvation.

I also observe that ROX1-YFP is expressed at much lower level in HEM1::LEU2 background than in HEM1+ background and that the range of expression over which ROX1 expression can be controlled is small (~2-fold).